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DEFINITION AND IDENTIFICATION OF VIRAL DNA VIRUSES OF THE FAMILY HERPESVIRIDAE IN ORAL FLUID OF PATIENTS WITH INFLAMMATORY AND DYSTROPHIC-INFLAMMATORY PERIODONTAL DISEASE, ASSOCIATED WITH PERSISTENT HERPES VIRUS INFECTION

Authors: Volosovets T.N.

Pages: 199-208

Abstract

Introduction. Lesions of periodontal herpesvirus is a complex process and is carried out by direct viral infection and replication, or due to virus-induced changes in the immune system. The prevalence of dystrophic and inflammatory lesions parodonta in the population; the complexity in the implementation of prevention and treatment of disease, and the ambiguity in the interpretation of the basic pathogenetic mechanisms (interrelated inflammatory, immune and metabolic) makes this issue extremely important in medicine.

Our aim was to detect viral DNA viruses of the family Herpesviridae in oral fluid of patients with inflammatory and dystrophic-inflammatory periodontal disease, associated with persistent herpes virus infection.

Material and methods. PCR and ELISA was performed detection and identification of viral DNA viruses of the family Herpesviridae in oral fluid of patients with inflammatory and dystrophic-inflammatory periodontal disease, associated with persistent herpes virus infection. I examined 290 patients with inflammatory and dystrophic-inflammatory periodontal disease (catarrhal gingivitis, generalized periodontitis (CP) and the initial and degree) and, depending on the presence or absence of persistent HVI divided them into groups.

Within the study was conducted immunological study of 170 patients with pathology of periodontal tissues infected by viruses of the family Herpesviridae (Experimental (I) group).

Results. As a result of the survey it was found that 170 (52.79 %) patients had relatively persistent HVI of virus and made D (I) group. 42.21% of patients had persistent HVI available and made the comparison group (II).

Conclusions

    1. During the polymerase chain reaction (PCR) in oral fluid of 170 patients of the main group reproduction HVI was detected only in 38.82 % of subjects: the herpes simplex virus (HSV) 1 and 2 – in 26.46 % of patients;–; the cytomegalovirus (CMV ) – in 10.58 % of patients. With greatest frequency in oral fluid was observed reproduction HSV 1 and type 2.
    2. Evaluating the local immunity by ELISA, IgM class antibodies to HSV RR was found in ; on EBV – in 3.77% of patients; to CMV – in 3.53 % of individuals.
    3. IgG class antibodies to EBV were determined in 51.76 % of patients; to CMV – in 68.23 % of patients; to HSV 1 and 2 – in 88.23 % of patients.

Key words: salpingoophoritis, inflammation, adolescents, IL-1β gene 511C/T.

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