Authors: Smiyan O.I., Kurganska V.A., Romaniuk O.K., Sichnenko P.I., Bynda T.P., Gorbas V.A., Vasylieva E.G.
Introduction. Dysbiotic violation of intestinal microflora lead not only to changes in metabolism, but also can be a direct cause of a number of pathologies, including allergic, increase the risk of disease, including bronchial asthma.
The purpose of our study is to determine the effect of probiotic medicine on the condition of the intestinal microflora of children with asthma and indirect - on the immune system and trace element metabolism.
Materials and Methods. The study involved 43 children with moderate persistent form of bronchial asthma between the ages of 12 and 18 to identify the influence of probiotic medicine on the state of the biocenosis of the intestine, the immune system and the trace element composition. To determine the condition of the intestinal microbiota performed microbiological examination of excrement for determining species composition and population of microorganisms by the method of Epstein-Litvak RB and Vilshanska FL. The study of the immunological status of children was conducted by determining the level of indicators of cellular and humoral immune system (CD3 +, CD4 +, CD8 +, CD16 +, CD22 +, FI, IgG, IgA, IgM, IgE), and the profile of cytokines (proinflammatory IL-1 and anti-inflammatory IL- 4) in immunological laboratory "Plus Diagnostics" on immunoferment analyzer "ImmunoChem - 2100 MicroplateReader" firm "HighTechnology, Inc." (USA), 2009 model year. The explore of trace-element composition of blood serum was based on the determination of concentrations of Zn, Cu, Mg and Ca by atomic absorption spectrophotometry. Statistical analysis of the results was performed according to the method of variation statistics using licensing program Microsoft Excel 7.0. To estimate the density of communication among the variables have used the correlation coefficient, the significance of which tested for appropriate formula and compared with tabulated (t Student”s test ).
Discussion. The dependence of the normalization of intestinal microbial spectrum not only on the nature of the treatment, in particular the inclusion of complex treatment probiotic preparation " Laktofit " that contains a combination of lactic acid bacteria (Lactobacillus Sporogenes and Bacillus coagulans), Folic acid (vitamin B9 ), and Cyanocobalamin (vitamin B12 ), but also depends on the dose. Administration of a double dose also gave a more pronounced effect on the impact of cellular and humoral immunity, revealed a decrease in the production of proinflammatory cytokines and normalization of the mineral balance in this group of children.
The obtained results gives reason to recommend the inclusion of probiotic preparation in complex therapy of moderate persistent forms of asthma in older children from the first days after hospitalization for acute illness according to this scheme :- 2 capsules 2 times daily with meals for 14 days.
Keywords: children , asthma, probiotic , microbiocenosis intestine, immune system, microelements.