Introduction. Transmembrane protein ANKH inorganic pyrophosphate transport regulator (ANKH) is regulates transport of inorganic pyrophosphate (PPi) of the cells into the extracellular environment, thus by playing an important role inhibitor of ectopic calcification of the vascular wall. Protein is contains 7-12 multi-pass transmembrane domains, each of 20 amino acids long. Mutations in this gene have been associated with autosomal dominant craniometaphyseal dysplasia and calcium pyrophosphate dehydrates deposition disease. Persons with recessive mutation of the ANKH gene are suffering from progressive ankylosis with concomitant vascular calcification caused by violation of inorganic pyrophosphate transport. As the calcification of the atherosclerotic plaque is an unto ward prognostic factor of the acute coronary syndrome, the polymorphism of the ANKH gene can be associated with the disease progression.
Purpose. To establish the frequency of allelic variants of the ANKH gene for T134967G polymorphism in patients with acute coronary syndrome (ACS) in persons with normal blood pressure and hypertension.
Materials and Methods. We used venous blood of 118 patients with ACS (22% women and 78% men) aged 40 to 73 years (mean age 55,9±0,89 years) who was hospitalized in the cardiology department of Sumy City Clinical Hospital #1. The control group consisted of 110 patients. Definition of T134967G polymorphism (rs187483) of ANKH gene was performed using PCR with the following restriction fragment length analysis of the allocation of them by electrophoresis in agarose gel. Restriction Hin6I (HinP1I) was used for restriction analysis. Statistical analysis was performed by using the software package SPSS-17. Thus the significance of differences was determined by Pearson’s chi-squered test and Student’s t-test. The value of P < 0,05 was considered as significant.
Results. Genotyping of patients with ACS for T134967G polymorphism of the ANKH gene was allowed setting the frequency with which there are some variants of this gene in patients with normal values of blood pressure (BP) and hypertension. The distribution of allelic variants among patients with ACS with different values of blood pressure was not significantly different (P = 0,949).
In persons who have the T/T genotype in control group and in patients with ACS, the value of BP syst. (P = 0,623), BP diast. (P = 0,065), BP pulse (P = 0,265) and BP mean (P = 0,159) were not significantly different. Values of BP syst. (P = 0,839), BP diast. (P = 0,561), BP pulse (P = 0,407) and BP mean (P = 0,827) in carriers of minor allele (T/G+G/G) were not significantly different between controls and patients with ACS. Patients with ACS – homozygotes for the major allele (T/T) – had significantly higher blood pressure syst. (P <0,0001), BP diast. (P <0,0001), BP pulse (P = 0,0007) and BP mean (P <0,0001) than practically healthy persons.Values of BP syst. (P <0,0001), BP diast. (P = 0,0003), BP pulse (P = 0,0001) and BP mean (P <0,0001) in patients with ACS and genotype T/G+G/G for T134967G polymorphism of the ANKN gene were also significantly higher than in controls.
Conclusion. In patients with ACS, regardless of genotype (T/T or T/G+G/G) values of BP syst., BP diast., BP pulse and BP mean is significantly higher than in the control group.
Key words: ANKH inorganic pyrophosphate transport regulator, acute coronary syndrome, allelic polymorphism.
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