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COMPARATIVE EVALUATION OF THE MAIN HEMATOLOGICAL INDICES OF VENOUS AND CAPILLARY BLOOD FROM DONORS IN SAMPLES OBTAINED USING K2EDTA AND K3EDTA

Аuthors: S. M. Dmytruk, S. I. Kryvtsun, I. B.Batura, S. A. Dmytruk

Pages: 555–563

Abstract

Relevance of carrying out a comparative analysis of hematological parameters in venous and capillary blood samples which were obtained using K2EDTA and K3EDTA is based on the need to take into account possible differences in the standardisation of pre-analytical phase of laboratory research, validation of analytical methods, the choice of biological material for analysis, comparing the results of the laboratory studies performed using venous and capillary blood.
Comparative analysis included analysis of the number of white blood cells, red blood cells, platelets, hemoglobin and hematocrit value in samples of venous and capillary blood taken using K2EDTA or K3EDTA, and was investigated by an automatic hematology analyzer in whole blood analysis mode.
Capillary blood samples taken using K2EDTA or K3EDTA in comparison with venous blood samples established increasing number of leukocytes, lower level of hemoglobin, smaller value of hematocrit, smaller number of erythrocytes and platelets. These differences, with an exception to the composition of capillary blood, are due to: greater sensitivity of procedure of sampling capillary blood to the factors that affect the value of pre-analytical phase error and depend on the type, brand and state of aggregation of anticoagulant, which are used in test tubes. In the data analyzed it appears that K2EDTA is an anticoagulant of choice during the selection and preparation of capillary blood samples for hematology research procedures.
Keywords: white blood cells, red blood cells, platelets, hemoglobin, hematocrit, venous blood, capillary blood, EDTA.

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