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IMPACT OF BIOFLAVONOIDS ON HEART BIOCHEMICAL INDICES IN MALE AND FEMALE RATS WITH HYPERHOMOCYSTEINEMIA.

Аuthors: A. V. Melnik

Pages: 856–862

Abstract

         

Introduction. Hyperhomocysteinemia (HHC) is a recognized independent risk factor for cardiovascular disease. One of the biochemical mechanisms of cardiotoxic action of excess homocysteine ​​is the development of oxidative stress. Today, such drugs as bioflavonoids (genistein, quercetin, etc.) are increasingly used for cardioprotection which exhibit antioxidant and anti-inflammatory properties. Along with this, it is shown that genistein also reveals hypohomocystinemic effect on acute methionine HHC. However, bioflavonoids’ ability to adjust the disruption of free radical processes in the myocardium of male and female rats under conditions of HHC remains unexplored.

The aim of the study was to explore the effect of bioflavonoids quercetin and genistein which induce hyperhomocysteinemia damage to cardiomyocytes and changes in the state of antioxidant systems of the heart in male and female rats.

Materials and methods. Experiments were performed on 80 white laboratory male and female rats (220–280 g). For hyperhomocysteinemia model of rats, D, L-homocysteine thiolactone (intragastral 100 mg/kg a day, 28 days,) in 1 % starch solution was used. Some animals in addition to homocysteine thiolactone was treated with genistein (2.5 mg / kg body weight i.g.) or quercetin (25 mg / kg body weight i.g.) for 28 days.

In myocardium, NADPH-oxidase activity, superoxide dismutase, content of malondialdehyde, protein carbonyl groups was determined. In blood serum, content of homocysteine, aspartatе aminotransferase activity and creatine phosphokinase was evaluated. Statistical processing of the results was performed using the program Statistica SPSS 17.0.

Results and discussion. Applied pharmacotherapy prevented decrease in activity of superoxide dismutase and glutathione deficit under the conditions of HHC in male and female rats, the mentioned action is more expressive under genistein. In addition, the introduction of bioflavonoids inhibits the growth of oxidized glutathione, activation of NADPH-oxidase and processes of free radical oxidation of lipids and proteins in male and female rats, but the effectiveness of genistein was higher than of quercetin. Under these conditions, genistein showed more expressive, membrane-stabilizing effect on cardiomyocytes in males and females than quercetin.

Conclusion. It was established that under the conditions of HHC applying of genistein only constrains effectively the development of oxidative stress in male and female rats.

Keywords: hyperhomocysteinemia, oxidative stress, myocardium, genistein, quercetin, sex.

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References

  1. Lai WK, Kan MY. [Homocysteine-Induced Endothelial Dysfunction]. Ann Nutr Metab. 2015;67(1):1-12.
  2. Wenzel U, Fuchs D, Daniel H. [Protective effects of soy-isoflavones in cardiovascular disease: identification of molecular targets]. Hamostaseologie. 2008;28(1-2):85-88.
  3. Han S, Wu H, Li W, Gao P. [Protective effects of genistein in homocysteine-induced endothelial cell inflammatory injury]. Mol Cell Biochem. 2015;403(1-2):43-49.
  4. Zhen P, Zhao Q, Hou D, Liu T, Jiang D, Duan J, Lu L, Wang W. [Genistein attenuates vascular endothelial impairment in ovariectomized hyperhomocysteinemic rats]. J Biomed Biotechnol. 2012; Retrieved from: http://dx.doi.org/ 10.1155/2012/730462
  5. Stangl GI, Weisse K, Dinger C, Hirche F, Brandsch C, Eder K. [Homocysteine thiolactone-induced hyperhomocysteinemia does not alter concentrations of cholesterol and SREBP-2 target gene mRNAS in rats]. Exp. Biol. Med. 2007;232(1):81-87.
  6. Roslan J, Giribabu N, Karim K, Salleh N. [Quercetin ameliorates oxidative stress, inflammation and apoptosis in the heart of streptozotocin-nicotinamide-induced adult male diabetic rats]. Biomed Pharmacother. 2017;86:570-582.
  7. Zaichko NV, Olhovskiy AS, Melnik AV. Yurchenko PA, Grigoreva AS, Konahovich NF. [Effect of the polymicroelement drug "esmin" on aging changes of hydrogen sulfide content and markers of pro-antioxidant system in the myocardium of rats]. Ukrainian Biochemical Journal. 2014;86(3):69-76
  8. Miller AA, De Silva TM, Jackman KA, Sobey CG. [Effect of gender and sex hormones on vascular oxidative stress]. Clin Exp Pharmacol Physiol. 2007;34(10): 1037-1043.