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CORRECTION OF THE STRUCTURAL CHANGES OF THE FUNDAL PART OF STOMACH CONDITIONED BY THE GENERAL AND INTRACELLULAR DEHYDRATION OF THE ORGANISM WITH ANTIOXIDANT AND MEMBRANE PROTECTIVE DRUGS

Аuthors:  V. I. Hula

Pages: 330–340

Abstract

         

This article is devoted to studying the morphological changes of structures of the stomach fundus on the macro- micro- and ultrastructural levels of the organization under conditions of different types and degrees of dehydration on the organism of laboratory rats and the possibility of correction of the morpho-functional state of the stomach tissues with using of ethylmethyldisopyridine succinate.

The purpose of the research was to study histological, histochemical, immunohistochemical, ultramicroscopic, and morphometric changes of the stomach fundus structures of laboratory rats in conditions of general and intracellular dehydration of the organism and to determine the possibility of their pharmacological correction with the membrane protective and antioxidant properties (2-ethyl-6-methyl-3-hydroxypyridine succinate).

Materials and methods. 36 white male laboratory rats of mature age and 150–190 g of weight were used for the research. Animals were divided into control and experimental series. The experimental series consisted of 4 groups of 6 rats in each of them. In the first group of animals the general dehydration of the sublethal degree of gravity was created. The second group of rats was in similar conditions, but throughout the experiment of general dehydration they received the intramuscular injections of ethylmethylhydroxypyridine succinate.

Severe intracellular dehydration conditions were created for the third group of rats. The fourth experimental group of rats consisted of 6 animals that were under similar conditions of intracellular dehydration of the body but they received an ethylmethylhydroxypyridine succinate throughout 30 days of this experiment. The control series consisted of 2 groups of animals that were on the usual drinking and feeding diet and were excluded from the experiment on the 9th day (6 rats) and the 30th day (6 rats) of the research. Experimental animals were also excluded from the research according to the indicated terms. The histological, histochemical, immunohistochemical, ultramicroscopic and morphometric study of the structures of the rat`s stomach wall in fundal part under that conditions, as well as statistical analysis of the obtained results was conducted.

Results. The obtained results show the functional ability of mucocytes of the stomach to form a mucous layer on the surface of the mucous membrane of the stomach, to preserve the structural organization of the glands, to reduce the quantitative losses of cells and their destructive-dystrophic changes, to improve the reparative ability and to reduce the intensity of microcirculatory disorders.

Conclusion. It has been discovered that usage of ethylmethylhydroxypyridine succinate is effective for maintaining the vital functions of the fundus of the stomach wall, depending on the type of dehydration.

Keywords: stomach, fundal part, rats, general dehydration, intracellular dehydration, correction, ethylmethylhydroxypyridine succinate.

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