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STIMULATION OF REPARATIVE REGENERATION OF THE YOUNG RAT’S TIBIA INJURED BY SEVERE DEHYDRATION

Authors: Ohienko M.M., Bumeister V.I.

Pages: 30-38

Abstract

In modern life trauma, along with cardiovascular and oncological diseases, is one of the three causes leading to mortality. Our aim was to study the reparative regeneration process inyoung animals (control group);in animals with stimulated severe degree of dehydration (experimental group) and general conditions to correct morphological changes of the injured tibiae caused by severe degree of dehydration (applying "SOLCOSERYL" drug).The experiment was conducted on young male rats aged 4 months, which were divided into 2 groups: control and experimental.The experimental group was subdivided into two subgroups: the rats with severe degree of dehydration (I) and the ratswhich underwent the corrective treatment of structural changes caused by severe degree of dehydration applying "SOLCOSERYL"drug (II). After 3 days there were changes in the cellular composition in the animals from the subgroup I. On the 15th day, severe hematoma were determined in the immature areas of granulation tissue. We observed that the area of fibroreticulate tissue increased, but the area of coarse-fibered bone tissue reduced by 15.48 % (p0.0001) compared with the control group. After 15 days in the rats which underwent correction of structural change occured a positive tendency in histostructure: coarse-fibered bone tissue area decreasedby 16.10 % (p ≤ 0.0001), and the plate – up to 14.33 % (p ≤ 0.0001) compared with animals with severe degree of dehydration. After 24 days the structure of theregenerateschanged dramatically in the animals with severe degree of dehydration. For example, the area of coarse-fibered bone tissue increased up to 8.05 % (p ≤ 0.0001) and lamellar bone reduced by 22.82% (p0.0001) comparing with the control group. After 24 days firbroreticulate tissue was formed in the animals which underwent corrective treatment.There was more lamellar tissue in the animals which underwent corrective treatment comparing with the animals which had water imbalance,by 24.75 % (p ≤ 0.0001). Accoding to the histological investigation, the results demonstrated that severe dehydration did not change the order of reparative osteogenesis stages, but delayedthe restoration of bones. Thus, the corrector had positive effect on the reparative osteogenesis. Applied "SOLCOSERYL"drug, as a corrector,eliminated negative dehydration and accelerated restructuring and maturation of bone structures.

Key words: general dehydration, cellular composition, correction, "SOLCOSERYL", reparative regeneration.

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