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DETERMINATION OF THE HERPESVIRIDAE FAMILY OF VIRUSES IN SAMPLES OF PERIODONTAL TISSUES AND STUDY OF IMMUNOLOGICAL PARAMETERS IN PATIENTS WITH INFLAMMATORY AND DYSTROPHIC-INFLAMMATORY PERIODONTAL DISEASE, ASSOCIATED WITH PERSISTENT HERPES VIRUS INFECTION

Authors: Volosovets T.N.

Pages: 80-87

Abstract

       

Introduction. Herpesvirus infection (HVI) is a common viral infections of man. There are people in all countries, in all climatic and geographical zones, with a variety of clinical manifestations that are sometimes very dangerous to human life. Lesions of periodontal herpesvirus is a complex process. It is carried out by direct viral infection and replication, or due to virus-induced changes in the immune system.

Purpose.Our aims were to determine the Herpesviridae family of viruses in samples of periodontal tissues and study immunological parameters in patients with catarrhal gingivitis (generalized periodontitis of initial and first degree), associated with persistent herpes virus infection.

Material and methods. Determination of the presence of pathogens in tissues taken from pathological focus was conducted by the own- technique. We took 87 samples of periodontal tissues removed from the core (I) group who had a history of herpes signs of infection (64 people) and a comparison group of patients (II), they had existing displays of herpesvirus infection (23 people), which was a complete dental health with removing roots (they are not subjectsof further orthopedic treatment).

We studied the local cellular and humoral immunity periodontal tissues analyzing content subpopulations of T lymphocytes and cytokines in seized samples of periodontal tissues .

Results. Determination of the presence of pathogens by PCR in tissues taken from the pathological center was used for early and accurate diagnosis and the general availability of herpesvirus infection in cell suspensions of periodontal tissue.

The proposed method can improve the diagnosis reliability and efficiency of viral lesions of periodontal tissues , and obtain information about the presence of a viral pathogen within 8 hours. However, using this method enables to reduce the amount of samples to 0.06 ml, and incubation duration to 8 hours. There are studies on the molecular level to enhance the reliability of diagnosis of infection. They are are connected with the assessment of the risk of destruction of bone of the alveolar process in inflammatory and dystrophic- inflammatory periodontal diseases; they define the role of viruses in immunogenetic determination of microorganism in the development and progression of the disease.

Conclusions. The herpes virus can multiply in the tissues and parodonta mucosa and gums, usually reaching greater concentration in tissues upper-gingival than under-gingival areas. They were found in biota samples of normal tissues parodonta .

That’s why, we assumed that viral and bacterial colonization not only ran, but also strengthened the parodontal tissue inflammation..

Key words: PCR, herpes infection, herpes simplex virus (HSV), cytomegalovirus (CMV), Epstein - Barr virus (EBV), periodontal tissues.

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