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GENETIC MARKERS OF APPENDAGES INFLAMMATION IN THE PUBERTY AGE GIRLS

Authors: Humenna K.Yu., Sydorchiuk L.P., Andriets O.A.,  Bodnariuk O.I.

Pages: 118-126

Abstract

      

Introduction. Late diagnosis and treatment of acute appendages inflammation in the puberty age girls are key causes of menstrual irregularities, recurrent exacerbations and frequent appearance of complications, chronic pelvic pain, possible ectopic pregnancy in future or infertility, endocrine and immunological disorders. The complex causes of tubes and ovarian inflammation in teenagers involves both specific (infectious) and non-specific factors, including genetic. Genetically caused dysregulation of the inflammatory response at the place of damage may be the result of point mutations in genes of the interleukins (IL) family. Clinical interest shows the mutation at 511 position of the IL-1β gene promoter (located at chromosome locus 2q14 (q13-21).

Our aim wasto analyze the association of 511C/T polymorphism of IL-1β gene (id.:rs16944) with salpingoophoritis in early (1214 years) and late (1518 years) adolescence.

Materials and Methods. 88 screened patients with salpingoophoritis: with specific (59.1%) and with non-specific appendages inflammation (40.9%);early puberty age (34.1%; 12–14 years old), late puberty age (65.9%, 15–18 years old), and 31 healthy persons with respective age participated in prospective study. Alleles of polymorphic locus were studied by polymerase chain reaction based method. Alleles' discrimination of IL-1β gene was performed by restriction endonuclease Aval ("Fermentas", Lithuania). Statistical processing was performed with Statistica® 7.0 (StatSoft Inc., USA) software. The differences were considered significant at p < 0.05.

Results. Among patients with nonspecific salpingoophoritis the T-allele frequency dominates the CC-genotype by 4.99 times. In adolescent girls 1518 years old with salpingoophoritis prevail mutant T-allele of IL-1β gene (id.:rs16944) over the C- allele in the ratio of 72:44 (p = 0.041); at the age of 1214 years the ratio was 38:22 (p > 0.05). Favorable CC-genotype met in every fifth teen with salpingoophoritis (20.5 %) and almost in every second (41.9 %) of the control group. The CT-genotype was recorded in almost every third girl (34.7 % in the study group, 38.7% among healthy subjects). "Unfavourable" TT- genotype was present in almost half of the individuals of the study group (45.4 %) and only one in five healthy adolescents (19.4 %). Specific salpingoophoritis met by 2.22 times more often in CC-genotype carriers girls aged 1518 years than in those of 1214 years (c2= 5.88, p = 0.049). Nonspecific salpingoophoritis opposite occurred less frequently by 3.15 times in CC-genotype carriers aged 1518 years than in adolescents with the same genotype of 1214 years (c2= 7.22, p = 0.031).

The presence of T allele or TT genotype of IL-1β gene increases the predisposition risk for tub-ovarian inflammation, regardless of their specificity, in girls of pubertal age (especially 1518 years old) by 1.542.58 times [OR = 2.344.17, OR 95 % CI = 1.0712.6 p ≤ 0.0320.002]. C-allele and CC genotype play a protective role and show the lowest probability of appendages inflammation, especially nonspecific salpingoophoritis [OR = 0.280.43, p ≤ 0.0220.001].

Conclusion: T-allele of IL-1β gene C-511T polymorphism associate with tub-ovarian inflammation in the puberty age girls.

Key wordssalpingoophoritis, inflammation, adolescents, IL-1β gene 511C/T.

 

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