Get Adobe Flash player


Authors: Romaniuk A.M., Moskalenko Yu.V., Shkandala A.Yu.

Pages: 39-49


The researchpresents the results of the study of seminal gland tissue of 128 immature rats treated with salts of zinc, copper, iron, manganese, chromium and lead from drinking water for 60 days. The aim of the study was to investigate the accumulation of zinc, copper, lead, manganese, chromium and iron in the tissue of the testes during their alimentary intake and under conditions of L-carnitine correction. Using spectrophotometry in atomic absorption mode, it was found out that the level of zinc reduced and the content of other analyzed trace elements increased. Accumulation of copper, iron, manganese, chromium and lead in seminal gland tissue was mostly expressed in the period of 60 days; iron, lead and chromium showed the largest organ tropism. The low rate of HMC accumulation in the testes of rats of breast and suckling age periodscould be explained by the limited intake of xenobiotics via maternal milk.Loss of zinc by testis tissue was caused by antagonistic interactions between chemical elements that came into the body of rats in excessive quantities. Interaction of chemical elements at different levels could lead to secondary violations of chemical and structural homeostases of the organ causing further inhibition of its function.

The results of chemical analyses of testes of immature rats, obtained by SEM, indicatedthe dependence of accumulation of micro elements of heavy metals from the morphofunctional activity of the histological structure of the studied organ. Heavy metals mainly accumulated in the functionally active spermatogenic epithelium.

Key words: testes, immature rats, heavy metals, chemical composition, atomic absorption spectroscopy, scanning electron microscopy with microanalysis.

  * This email address is being protected from spambots. You need JavaScript enabled to view it.

The full text

To view the full text


  1. Grytchuliak BV, Grytchuliak VB, Hallo OY. [Histoultrastructure of convoluted tubule of testis after plastic of inguinal canal wall]. Clin anat and topogr. surg. 2009;8(4):43–
  2. Yvasiuk YJ. [Electron microscopy characteristic of testes after mechanistic injury]. Electro-microscopicheskaja characteristica yayechok pislya mechanichnoyi travmy:materialy naukovo-praktychnoi konferencii [Applied aspects of morphology]. Ivano-Frankivsk, 2010, pp. 64– (In Ukrainian).
  3. Kartavceva OV. [Systematic analisis of influence mother social stress in first trimester of pregnancy on hormonal regulation of spermatogenic function in male progeny]. World of medicine and biology. 2009;2:80–
  4. Narbutova ТЕ. [Dinamic structure-fuctional changes of testes mouse second progeny under cumulation of lead and alfa-tocoferol treatment]. Biomedical and Biosocial Anthropology. 2011;16:27–31.
  5. Romanyuk AM, Saulyak SV, Moskalenko RA, Moskalenko YuV. [Spermatogenic function under the influence of heavy metals salts and correction of preparation Tivortin ®]. Lik. sprava. 2012;1–2:123–
  6. Zhou X, Liu F, Zhai S. Effect of L-carnitine and/or L-acetyl-carnitine in nutrition treatment for male infertility: a systematic review. Asia Pac. J. Clin. Nutr. 2007;16 (1):383–390.
  7. Verkhovna Rada Ukrainy [The Verkhovna Rada of Ukraine]. Retrieved from:
  8. Zapadniuk VY, Zapadniuk YP, Zachariia EA, Zapadniuk BV. Laboratornye zhyvotnye. [Experimantal animals]. Кyiv: Vyscha shkola Publ., 1983, 383 p.
  9. PogorielovMV, BuymesterVI, TkachHF, BonchevSDetal. Macro- i microelementy (obmin, patologiia, metody vyznachennya[Macro-andtraceelements(metabolism, pathology,methodsdetermination)].Sumy:SSU, 2010, 147p.
  10.  Babekov IM, Asadov ChD, Stryzhkov NA. [Effect of low-intensity laser radiation on the seminiferous tubules and sperm] Laser medicine. 2007;11(1):18–21.
  11. RomanyukAM, Saulyak SV, Moskalenko RA, Moskalenko YuV, Budko AYu. [Microelementstatuschanges of rats’ testis under increased intake of heavy metals salts]. Morphology. 2011;5(2):55–60.
  12. Skalniy AV, Rudakov I.A. Bioelementy v medicine [Bioelements in medicine]. Мoscow: Onyx 21 Century Publ., 2004, 272 p.
  13. Lopez Alonso M, Prieto Montaña F, Miranda M. Interactions between toxic (As, Cd, Hg and Pb) and nutritional essential (Ca, Co, Cr, Cu, Fe, Mn, Mo, Ni, Se, Zn) elements in the tissues of cattle from NW Spain. Biometals. 2004; 17(4):389–97.
  14. Rawy SM, Seif Al Nassr FM. Zinc sulphate and vitamin E alleviate reproductive toxicity caused by aluminium sulphate in male albino rats. Toxicol Ind Health. 2013;1:24–9.
  15. Yamaguchi S, Milura C, Kikuchi K. Zinc is an essential trace element for spermatogenesis. Proc Natl Acad Sci U S A. 2009;106(26):10859-64. doi: 10.1073/pnas.0900602106
  16. Kakizaki I. Nukatsuka I, Takagaki  K. Effects of divalent cations on bovine testicular hyaluronidase catalyzed transglycosylation of chondroitin sulfates. Biochem Biophys Res Commun. 2011;406(2):239–44.
  17. Lavicolli I, Fontana L, Bergamaschi A. The effects of metals as endocrine disruptors. Journal of toxicology and environmental health. 2009;12:206–223.
  18. Schlorff EC, Husain  K, Somani  SM. Dose and time dependent effects of ethanol on antioxidant system in rat testes. Alcohol. 1999;2–3:203–14.